Anatomical characterization, histochemical and micromorphometric analyses of leaves of Caryocar brasiliense Cambess in different Cerrado physiognomies brasiliense Cambess in different Cerrado physiognomies

Barbara Helena Ramos, Kellen Lagares Ferreira Silva, Ronaldo Rodrigues Coimbra, Wagner Melo Ferreira


The aim of this study was to anatomically and histochemically characterize C.brasiliense leaves in three Cerrado physiognomies: dense, typical and thin, near the city of Porto Nacional, Tocantins, Brazil. Composite samples of leaves were collected, fixed in FAA and stored in 70% alcohol. Cross, longitudinal and paradermic sections of middle leaflets were obtained for making slides. For histochemical analyses, cross sections were obtained from newly collected, middle leaflets. The sections were subjected to reactions for detection of lipids, tannins, lignins and starch. The leaflets of C. brasiliense showed epidermis with bulky cells with thick cuticle on the adaxial epidermis and many multicellular trichomes present on the abaxial epidermis. The stomata are of a paracitic type, present ventrally in the dorsal in the abaxial epidermis. The mesophyll is dorsiventral, formed by two or three layers of palisade and spongy tissue. The vascular bundle of the main rib is of a collateral type and the terminations are characterized as vascular branching. The histochemical reactions were positive: lipids (cuticle), lignin (cell walls of vascular bundle fibers and vessel elements of the xylem) and tannins (idioblasts). Only the micromorphometric analyses revealed significant differences for most tissues measured in the different vegetation types.


Caryocar brasiliense,Lipids, Pequi, Tannins,Typical cerrado

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